During interphase (A), endogenous mCenp-F (green) and pocket proteins (p107, red) co-localize in nucleus and are excluded from nucleoli, DNA (To-ProIII, blue). At metaphase (B), mCenp-F and p107 are localized to areas that include the kinetochore (white arrow) at the outer edge of aligned chromosomes and appear to be associated with the mitotic spindle. During anaphase (C), the proteins become concentrated at the intracellular bridge and eventually become re-localized to daughter nuclei. Interestingly, mCenp-F and pocket proteins also co-localize at the midbody of dividing cells during cytokinesis (D, white arrowhead) where the remnants of polar microtubules and motor proteins are located.