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(A) Western blot analysis was performed to determine the protein levels of Bcl-2 and Bax in each group. GAPDH served as an internal control. (B) Caspase-3 and caspase-8 colorimetric assay kits were used to determine the activities of caspase-3 and -8 in each group. (C) Western blot analysis was used to determine the protein levels of cytochrome c in the cytosol in each group. GAPDH served as an internal control. **P<0.01. I/R, ischemia/reperfusion; GP, gypenoside.
