Table II.
Grouping | GAPDH Ct | TF Ct | ΔCt | ΔΔCt | 2−ΔΔCt |
---|---|---|---|---|---|
N | 32.95±0.55 | 23.87±0.04 | −9.08±0.39 | 0 | 1 |
NC | 32.77±0.03 | 23.85±0.32 | −8.92±0.23 | 0.15717 | 0.864346 |
TF-1 | 31.86±1.08 | 26.13±0.21 | −5.73±0.78 | 3.343803 | 0.483297a |
TF-2 | 33.37±0.17 | 26.17±0.47 | −7.2±0.37 | 1.875092 | 0.095393b |
TF-3 | 32.34±0.82 | 24.25±0.43 | −8.08±0.66 | 0.996823 | 0.310134a |
TF-4 | 32.59±0.64 | 26.90±0.07 | −5.69±0.45 | 3.389969 | 0.27261a |
ΔCt = TF Ct-GAPDH Ct, ΔΔCt = (TF Ct-GAPDH Ct) other samples - (TF Ct-GAPDH Ct).
P<0.05, compared with the blank control group and the negative control group;
P<0.01, compared with the blank control group and the negative control group.
TF, tissue factor; N, blank control; NC, negative control.