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. 2014 Feb 20;7(5):1065–1070. doi: 10.3892/etm.2014.1564

Figure 4.

Figure 4

Downregulation of MAPK phosphorylation by luteolin in LPS-stimulated BV2 microglia. BV2 microglial cells were treated with LPS (1.0 μg/ml) for the indicated time with or without pretreatment with 20 μM luteolin for 1 h. Cell lysates were prepared and used for western blot analysis with antibodies against the indicated MAPKs. Expression levels of phosphorylated MAPKs were quantified by OD ratios relative to controls, and these data are shown between the relevant blots. LPS rapidly activated MAPKs including p38, JNK, and ERK within 15 min of LPS stimulation, while pretreatment of cells with 20 μM luteolin markedly inhibited the LPS-induced phosphorylation of p38 and JNK. Data were collected from three independent experiments each carried out in triplicate. MAPK, mitogen-activated protein kinase; LPS, lipopolysaccharide; OD, optical density.