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. Author manuscript; available in PMC: 2014 Apr 18.
Published in final edited form as: J Pain. 2013 Feb 5;14(4):393–404. doi: 10.1016/j.jpain.2012.12.010

Figure 3.

Figure 3

Representative fluorescent photomicrographs of GFAP immunoreactivity in the vlPAG of animals treated with CFA+Morphine (a) and Handled+Morphine (b) (A). Densitometry of GFAP immunoreactivity in the vlPAG (B). Administration of morphine, in the absence of CFA (Handled+Morphine; n=7), resulted in a significant increase in GFAP immunoreactivity within the vlPAG (F3,22=10.022, p=0.0002). No differences in GFAP levels were noted for the CFA+Morphine (n=11), CFA+Saline (n=4) or Handled+Saline control groups (n=4). Relative band intensity of GFAP/β-actin in the vlPAG (C). Administration of morphine, in the absence of CFA (Handled+Morphine; n=5), resulted in a significant increase in relative band intensity of GFAP/β-actin in the vlPAG (F3,19=10.256, p=0.0003). No differences in GFAP levels were noted for the CFA+Morphine (n=5), CFA+Saline (n=7) or Handled+Saline (n=6) control groups.