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Densitometry of GFAP (A) and OX-42 (B) immunoreactivity in the vlPAG in Handled+Morphine (n=3), CFA+Morphine (n=4), Handled+Saline (n=4), and CFA+Saline (n=5) animals 24 hours following one morphine or saline injection. Neither CFA nor morphine (5 mg/kg, sc) increased vlPAG GFAP (F(3,12)=0.494; p=0.693) or OX-42 (F(3,12)=0.162; p=0.9198) levels in the vlPAG as compared to handled and saline controls.
