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. 2014 Apr 18;9(4):e92999. doi: 10.1371/journal.pone.0092999

Figure 5. GC-MS analysis of ergosterol isotopomers from Pichia pastoris unsaponifiable extracts.

Figure 5

A, TIC of an acetylated extract from cells grown in hydrogenated medium. The peak at RT = 36.10 min is ergosterol. B, TIC of an acetylated extract from cells grown in deuterated medium. The peak at RT = 35.26 min is deuterioergosterol. C, mass spectrum of ergosteryl acetate. Prominent ions and interpretation of the fragmentation pattern: M+(438), M+-acetate-H (378), M+-acetate-H-CH3 (363), M+-acetate-H-side chain (253). D, mass spectrum of deuterioergosteryl acetate. Prominent ions and interpretation of the fragmentation pattern: M+(481), M+-acetate-D (420), M+-acetate-D-CD3 (402), M+-acetate-D-side chain (278).