Figure 3. Trichophyton rubrum together with the cytokine combination IFN-γ/IL-17 synergistically induce RNase 7 and hBD-3 expression in keratinocytes.

(A) Primary keratinocytes were incubated with 1×10⁷/ml conidia of T. rubrum together with IFN-γ/IL-17A (each 20 ng/ml) for 24 h. Gene expression of RNase 7 (a) and hBD-3 (c) was analyzed by real-time PCR. Release of RNase 7 was determined by ELISA (b). Data are means ± SD (n = 3; *p<0.05, **p<0.01, Student's t test).