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. 2014 Apr 18;9(4):e95145. doi: 10.1371/journal.pone.0095145

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© 2014 Hu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunofluorescence analysis of desmin and α-SMA during the activation of PSCs (A) Immunofluorescence staining of desmin in day 1 (quiescent) and day 7 (fully activated) PSCs. (B) Immunofluorescence staining of α-SMA in day 1 (quiescent) and day 7 (fully activated) PSCs. DAPI (blue) was used to counterstain nuclei (magnification ×400).