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(A) PSCs were labeled with Annexin V for 15 min at room temperature with 5 µg/ml PI. Apoptotic rates were compared among PSCs treated with or without rmDkk-1 at different doses by flow cytometry. (B) Hoechst 33342 staining. (C) Analysis of Hoechst 33342 staining. Data are presented as mean±SD from three independent experiments. *p<0.05 compared with the PBS-treated group.
