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. 2014 Apr 18;9(4):e95145. doi: 10.1371/journal.pone.0095145

Figure 10. β-catenin-targeting siRNA inhibits the activation of PSCs in vitro.

Figure 10

PSCs were transfected with negative control and β-catenin siRNA. (A) Transfection efficiency of PSCs. (B) β-catenin and α-SMA levels in cells were measured by western blotting. Blots were re-probed for GAPDH to confirm equal protein loading. (C) Hoechst 33342 staining. (D) The levels of α-SMA, PDGFRβ, TGFβRII and collagen1α1 mRNA were measured by quantitative RT-PCR. Data are presented as mean±SD from three independent experiments. *p<0.05 compared with negative control. (E) Cell viability was assessed using the Cell Counting Kit-8 (CCK-8). (F) Analysis of Hoechst 33342 staining. Data are presented as mean±SD from three independent experiments. *p<0.05 compared with negative control.