Table 1. Primer Sequences Used for qRT-PCR Analysis.
Gene | Primer sequence (5'→3') | |
Desmin | Forward | TCTCTGAGGCTGAAGAATGGT |
Reverse | TCAATCTCGCAGGTGTAGGA | |
α-SMA | Forward | TGCCGAGCGTGAGATTGT |
Reverse | CCCGTCAGGCAGTTCGTAG | |
β-catenin | Forward | AGGGTGCTATTCCACGACTA |
Reverse | CACCCTTCTACTATCTCCTCCAT | |
Dkk-1 | Forward | TGAGGGCGGGAACAAGTA |
Reverse | TTCGGCAAGCCAGACAGA | |
Dkk-2 | Forward | ATGATGGAAACCTGGATTGGAA |
Reverse | GAGGCACATAACGGAAGCA | |
Dkk-3 | Forward | GGCAAGGGAATGTGGTAGAG |
Reverse | GTTGGGCGTTTGATTGTAGC | |
Dkk-4 | Forward | AGCACATAAATAAAGCCCAATCTC |
Reverse | ATAGTAGACGCAAAGCCAAAAGT | |
sFRP-1 | Forward | TCTTCCTCTGTTCGCTCTT |
Reverse | GGCTTCCGTGGTATTGG | |
sFRP-2 | Forward | GTCATGTCCGCCTTCG |
Reverse | TCGTCCTCATTCTTGGTTTT | |
sFRP-4 | Forward | TTTGTCACCTATCCCTCG |
Reverse | CCACTGTATGGACCTTCTAC | |
sFRP-5 | Forward | GGGGACCGAAAGTTGATT |
Reverse | GCCCGTCAGGTTGTCTA | |
Wnt1 | Forward | GCCCGCCTCCAGACTTATT |
Reverse | GTTCTGTGCTGCGGTTCT | |
Wnt2 | Forward | CGACACCCAGATGTGATGC |
Reverse | GGCCGATTCCCGACTACTT | |
Wnt3 | Forward | CCCTGCTCTGGATGGTGTA |
Reverse | GCCTGTTCTGTTGCGGTAG | |
Wnt3a | Forward | AATTTGGAGGAATGGTCTCTCGG |
Reverse | CAGCAGGTCTTCACTTCACAG | |
Wnt7a | Forward | GGCTTCTCTTCGGTGGTAGC |
Reverse | TGAAACTGACACTCGTCCAGG | |
Wnt8a | Forward | TTCGCAGGAGTGAAATCGG |
Reverse | AGCTGGGTGGAACGGTAT | |
Wnt8b | Forward | AATCACCCACATAAACCTTCCG |
Reverse | TCTTCAGTTCTCCCAATACCCAT | |
Wnt10b | Forward | GAAGGGTAGTGGTGAGCAAGA |
Reverse | GGTTACAGCCACCCCATTCC | |
Wnt4 | Forward | AGACGTGCGAGAAACTCAAAG |
Reverse | GGAACTGGTATTGGCACTCCT | |
Wnt5a | Forward | AGTCCTTTGAGATGGGTGGTA |
Reverse | CCTCTGGGTTAGGGAGTGTC | |
Wnt11 | Forward | CAGCCTTCGTGTATGCCCT |
Reverse | CCGTAGCTGAGGTTGTCCG | |
PDGFRβ | Forward | CCAGAAGTAGCGAGAAGC |
Reverse | ATCACCGTATCGGCAGTA | |
TGFβRII | Forward | TTTCGGAAGAATACACCAC |
Reverse | GACACGGTAGCAGTAGAA | |
Collagen1α1 | Forward | CGCCATCAAGGTCTACTG |
Reverse | ACGGGAATCCATCGGTC |