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. 2014 Apr 18;9(4):e95145. doi: 10.1371/journal.pone.0095145

Table 1. Primer Sequences Used for qRT-PCR Analysis.

Gene Primer sequence (5'→3')
Desmin Forward TCTCTGAGGCTGAAGAATGGT
Reverse TCAATCTCGCAGGTGTAGGA
α-SMA Forward TGCCGAGCGTGAGATTGT
Reverse CCCGTCAGGCAGTTCGTAG
β-catenin Forward AGGGTGCTATTCCACGACTA
Reverse CACCCTTCTACTATCTCCTCCAT
Dkk-1 Forward TGAGGGCGGGAACAAGTA
Reverse TTCGGCAAGCCAGACAGA
Dkk-2 Forward ATGATGGAAACCTGGATTGGAA
Reverse GAGGCACATAACGGAAGCA
Dkk-3 Forward GGCAAGGGAATGTGGTAGAG
Reverse GTTGGGCGTTTGATTGTAGC
Dkk-4 Forward AGCACATAAATAAAGCCCAATCTC
Reverse ATAGTAGACGCAAAGCCAAAAGT
sFRP-1 Forward TCTTCCTCTGTTCGCTCTT
Reverse GGCTTCCGTGGTATTGG
sFRP-2 Forward GTCATGTCCGCCTTCG
Reverse TCGTCCTCATTCTTGGTTTT
sFRP-4 Forward TTTGTCACCTATCCCTCG
Reverse CCACTGTATGGACCTTCTAC
sFRP-5 Forward GGGGACCGAAAGTTGATT
Reverse GCCCGTCAGGTTGTCTA
Wnt1 Forward GCCCGCCTCCAGACTTATT
Reverse GTTCTGTGCTGCGGTTCT
Wnt2 Forward CGACACCCAGATGTGATGC
Reverse GGCCGATTCCCGACTACTT
Wnt3 Forward CCCTGCTCTGGATGGTGTA
Reverse GCCTGTTCTGTTGCGGTAG
Wnt3a Forward AATTTGGAGGAATGGTCTCTCGG
Reverse CAGCAGGTCTTCACTTCACAG
Wnt7a Forward GGCTTCTCTTCGGTGGTAGC
Reverse TGAAACTGACACTCGTCCAGG
Wnt8a Forward TTCGCAGGAGTGAAATCGG
Reverse AGCTGGGTGGAACGGTAT
Wnt8b Forward AATCACCCACATAAACCTTCCG
Reverse TCTTCAGTTCTCCCAATACCCAT
Wnt10b Forward GAAGGGTAGTGGTGAGCAAGA
Reverse GGTTACAGCCACCCCATTCC
Wnt4 Forward AGACGTGCGAGAAACTCAAAG
Reverse GGAACTGGTATTGGCACTCCT
Wnt5a Forward AGTCCTTTGAGATGGGTGGTA
Reverse CCTCTGGGTTAGGGAGTGTC
Wnt11 Forward CAGCCTTCGTGTATGCCCT
Reverse CCGTAGCTGAGGTTGTCCG
PDGFRβ Forward CCAGAAGTAGCGAGAAGC
Reverse ATCACCGTATCGGCAGTA
TGFβRII Forward TTTCGGAAGAATACACCAC
Reverse GACACGGTAGCAGTAGAA
Collagen1α1 Forward CGCCATCAAGGTCTACTG
Reverse ACGGGAATCCATCGGTC