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. 2014 Apr 18;9(4):e95130. doi: 10.1371/journal.pone.0095130

Figure 8. VAMP in phasic and tonic axonal terminals is susceptible to Clostridial neurotoxins under high frequency stimulation.

Figure 8

Immunostaining of VAMP and synapsin following the high frequency stimulation experiments using inactive and active TeNT-LC (A), BoNT/D-LC (B) and BoNT/B-LC (C). In A-C, arrows denote phasic terminals and arrow heads denote tonic terminals. Areas of overlap between VAMP and synapsin immunoreactivity are denoted in yellow in the merged image. Note that all three active neurotoxins reduced VAMP immunoreactivity. In A-C, no tonic terminals were present in the phasic image with active neurotoxin. Scale bars: 10 µm.