Skip to main content
. 2014 Apr 18;9(4):e94955. doi: 10.1371/journal.pone.0094955

Figure 3. SDS-PAGE analysis of β 2-AR solution.

Figure 3

Lane 1, collection from Ni2+ Sepharose 6 Fast Flow column eluted by 50% buffer B; Lane 2, protein molecular weight marker (the molecular weights from top to bottom are 116.0, 66.2, 45.0, 35.0, 25.0, 18.4 and 14.4 kDa, respectively); Lane 3–4, collection from Quaternary Sepharose Fast Flow column gradient eluted by 18%–40% buffer C; Line 5–9, collection from Quaternary Sepharose Fast Flow column eluted by 50%, 60%, 70%, 80% and 90% buffer C; Lane 10, β 2-AR supernatant.