Table 3. Results of artificial insemination of eggs derived from pure pejerrey mothers with sperm from three surrogate Patagonian pejerrey fathers (#1∼3; transplanted with pejerrey donor germ cells) and a control pure pejerrey father.
| Father | Time after GCT (months) | Number of eggs (n) | Fertilization rate (n; %) | Hatching rate (n; %) | Donor-derived germline transmission rate (n; %) |
| #1 | 7 | 160 | 158(98.7) | 150(94.9) | 19(12.6) |
| 11 | 140 | 131(93.5) | 122(93.1) | 30(24.6)* | |
| #2 | 7 | 155 | 147(94.8) | 141(95.9) | 56(39.7) |
| 11 | 170 | 162(95.2) | 150(92.5) | 44(29.3) | |
| #3 | 7 | 130 | 125(96.1) | 118(94.4) | 38(32.2) |
| 11 | 155 | 146(94.1) | 135(92.4) | 28(20.7)* | |
| Control | 7 | 140 | 132(94.2) | 125(94.6) | NA |
| 11 | 165 | 159(96.3) | 148(93.0) | NA |
Donor-derived germline transmission rates for each surrogate father were determined at 7 and 11 months after GCT; asterisks after the donor-derived germline transmission rate for 11 months indicate significant difference (Fisher's exact test) from the rate for the same father at 7 months. NA: not applicable