Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 18;9(4):e94266. doi: 10.1371/journal.pone.0094266

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Ryu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

90-nt homologous oligos incorporating a stop codon into the lacZ gene were electroporated into E. coli cells to analyze the recombination efficiency of the pRED integrated EcSIM strains as compared to EcNR2. Recombinants were selected on X-gal/IPTG plates. The recombination efficiency was calculated by estimating the fraction of white colonies in the total number of colonies.