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. 2014 Apr 18;9(4):e95118. doi: 10.1371/journal.pone.0095118

Figure 8. Yeast two hybrid analysis of PpKLR36, PpKLR39, PpKLR40, and PpKLR43 (AD) with PpXB3-1 and PpXB3-2 (DBD).

Figure 8

Y2H analysis of PpXB3-1 with PpKLR36, PpKLR39, PpKLR40, and PpKLR43 was performed three times and produced the same results. This figure shows a representative example. PpKLR kinases and PpXB3s were expressed with the activation domain (AD) and DNA-binding domain (DBD) in yeast, respectively. Detection of the interactions and elimination of false positive results required that the test was made more stringent by application of 40 mM 3-AT in the culture medium. (A) Yeast cells were spotted on SD-W, L, H medium plates. (B) Yeast cells were spotted on SD-W, L, H medium with 40 mM 3-AT plates.