Figure 5. Polymorph determination of calcium carbonate crystals modified by rhOtolin-1 alone or in combination with rmOC90.

Varying concentrations of rhOtolin-1 or rmOC90 protein, (A and B) 1000 nM rmOC90; (C and D) 667 nM rhOtolin-1; (E and F) 667 nM rhOtolin-1plus 500 nM rmOC90; (G) pure growth solution were dissolved in 7.5 mM CaCl₂ growth solution and crystals were grown for 48 hours by slow evaporation of NH₄HCO₃ into growth solution. Raman spectra were collected on pre-selected crystals of CaCO₃ with a HoloLab 5000 Raman microprobe spectrometer system.