Table 1.
Total lateral root length for cell wall mutants grown with and without parafilm to isolate shoots from mediaValues are mean±standard error. Asterisks indicate statistically significant deviations from wild type (P<0.05 using Student’s paired t-test). Total lateral root length for lrd5-2 and ray1 are significantly different from the lrd5-2/ray1 double mutant. AGP, arabinogalactan protein; NK, not known.
| Perturbed in: | Total lateral root length (cm) | |||
|---|---|---|---|---|
| Gene | Cell wall | Off parafilm | On parafilm | |
| Col | NK | NK | 0.18±0.08 | 0.10±0.05 |
| Cell wall mutants | ||||
| mur1 a | At3g51160 | Fucose | 2.27±0.24* | 1.65±0.26* |
| mur2 a | At2g03220 | Fucose | 0.01±0.00 | – |
| mur3 a | At2g20370 | Fucose | 0.41±0.16* | 0.24±0.15 |
| mur4 a | At1g30620 | Arabinose | 0.86±0.12* | 2.40±0.35* |
| mur5 a | NK | Arabinose | 0.13±0.05 | – |
| mur6 a | NK | Arabinose | 0.09±0.03 | – |
| mur7 a | NK | Arabinose | 0.05±0.02 | – |
| mur8 a | NK | Rhamnose | 1.23±0.19* | 0.73±0.34* |
| mur9 a | NK | Fucose, xylose | 2.94±0.28* | 0.09±0.04 |
| mur10 a | At5g17420 | Cellulose, fucose, arabinose, xylose | 0.11±0.03 | – |
| mur11 a,b | At3g59770 | Rhamnose, fucose, xylose, mannose | 0.47±0.10* | 0.70±0.30 |
| Cesa6 c | At5g64740 | Cellulose | 1.28±0.11* | 3.04±0.29* |
| cesa8 d | At4g18780 | Cellulose | 2.73±0.24* | 3.51±0.32* |
| xxt1/xxt2 e | At4g02500/At3g62720 | Xyloglucan | 4.13±0.36 | 2.43±0.58 |
| Family 77 GTs | ||||
| lrd5-2 | At2g35610 | Extensin arabinose | 0.77±0.11* | 0.80±0.22* |
| rra1 f | At1g75120 | Arabinose | 0.10±0.04 | – |
| rra2 f | At1g75110 | Arabinose | 0.07±0.03 | – |
| ray1 g | At1g70630 | AGP arabinose | 5.21±0.54* | 0.85±0.18* |
| lrd5-2/ray1 | At2g35610/At1g70630 | – | 5.16±0.42* | 2.12±0.28* |
a Reiter et al., 1997; b Austin et al., 2011;c Turner and Somerville, 1997; d Fagard et al., 2000; e Cavalier et al., 2008; f Egelund et al., 2007; g Gille et al., 2013