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. 2014 Mar 11;65(8):2219–2230. doi: 10.1093/jxb/eru099

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — AtRALF1 effect on BL-downregulated genes CPD (At5g05690) and DWF4 (At3g50660). (A, B) Semi-quantitative RT-PCR gene expression analyses performed in roots of 10-d-old AtRALF1-overexpressing (35S:AtRALF1) and AtRALF1-silenced (irAtRALF1) transgenic lines and wild-type plants (A) and quantitative qRT-PCR gene expression analyses (B). Error bars indicate SD. Columns followed by the same letter are not significantly different (P<0.01). (C) Semi-quantitative RT-PCR gene expression analyses performed in roots of _HisAtRALF1-treated 10-d-old wild-type plants. Total RNA was extracted from roots of plants after 30min of treatment with different concentrations of the peptide. GAPDH (At1g13440) expression was used as a control. All experiments were repeated at least three times (independent biological replicates).