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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Int J Cancer. 2014 Feb 20;135(1):48–60. doi: 10.1002/ijc.28662

Figure 5. FILIP1L down-regulates MMPs.

Figure 5

A, qRT-PCR analysis for MMPs conducted on cDNA from FILIP1L clone-derived tumors. The y axis represents fold change of DOX-induced tumors (n=3) over uninduced tumors (n=3), where each value was standardized with the housekeeping gene GAPDH. * indicates P<0.05. B, Immunofluorescent staining of MMP9 (green) in FILIP1L clone-derived tumors. Nuclei counterstained with DAPI (blue). A merged image is shown. Shown at right is the quantified data from four random fields per tumor (n=12). C, MMP9 activity of total tumor lysates from control and FILIP1L clones was measured using gelatin zymography. Each well represents a different tumor. The arrows indicate pro-MMP9 activity. The quantified data, where each pixel value of pro-MMP9 was standardized with that of GAPDH, is also shown. D, Gelatin matrix degradation in vitro from cultured control and FILIP1L clones was measured (n=50). E, Matrigel cell invasion assay 24 h after transfection of FILIP1L clones with control empty plasmid or plasmid encoding MMP9. Immunoblot analysis for MMP9 in the transfected cells is also shown. GAPDH blot is shown as the loading control.