Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2014 Feb 6;34(5):996–1001. doi: 10.1161/ATVBAHA.113.303113

Figure 2. Platelet-neutrophil/monocyte interactions.

Figure 2

Whole blood was collected from wild-type, hIL-4R/Ibα and VWF KO mice under static conditions. (A) Representative flow analysis of samples identifying the neutrophil population (Gr-1+/CD115−) within wild-type, hIL-4R/Ibα or VWF KO blood is shown. Gating exclusively onto a Gr-1+/CD115− population (upper left gray box in panel A) allowed for a second gating of associated CD41+ (platelet) events within the neutrophil gate. (B) The CD41+/Gr-1+/CD115− population analyzed as a percent of total Gr-1+/CD115− events illustrates platelet-neutrophil interaction based on GP Ib-IX expression. Wild-type and VWF KO express endogenous GP Ib-IX and are indiscernible from one another (p = 0.54) but both exhibit significantly higher platelet-neutrophil levels compared to hIL-4R/Ibα (p < 0.001). This establishes a GP Ib-IX role in augmenting platelet binding to neutrophils. (C) Utilizing CD115+ fluorescence to gate exclusively onto monocytes (lower right gray box in panel A) the GP Ib-IX dependent association with platelets is presented. Comparison of the sample groups showed the wild-type and VWF KO strains to be indistinguishable (p = 0.24). Blood from hIL-4R/Ibα mice had significantly lower platelet-monocyte associations relative to wild-type (p = 0.003). This indicates a significant GPIb-IX dependent modulation of platelet binding to monocytes in the circulation. N = 6 for each group.