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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Wiley Interdiscip Rev RNA. 2013 Dec 16;5(3):335–346. doi: 10.1002/wrna.1214

Table 1. Steady state levels of RNA oxidative damage in E. coli in response to H2O2 treatment.

Growth
condition		 8-oxoG/105
G* 		8-oxo-G RNA† Damaged RNA‡ Cell viability
(CFU)*
Normal aeration 1.0 ± 0.1 0.25% 2.4% (set to 100%)
Plus 1 mM H2O2 3.9 ± 0.1 0.98% 9.3% 83%
Plus 5 mM H2O2 10.9 ± 0.5 2.7% 24% 42%
*

8-oxo-G levels were determined 15 min after addition of H2O2 to exponentially growing cultures. CFU was determined after treatment with H2O2 for 60 min.

†

Based on the assumption that the length of RNA is 1 kb in average, the GC content is 50%, and 8-oxo-G is randomly distributed so that the percentage of 8-oxo-G containing RNA can be described by Poisson distribution.

‡

Based on the assumption that total damage occurrences can be 10 times of 8-oxo-G content.25 (Reference 25, p. 58, reproduced by permission of De Gruyter).