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. Author manuscript; available in PMC: 2014 Apr 18.
Published in final edited form as: Anal Chem. 2012 Aug 14;84(17):7495–7501. doi: 10.1021/ac301590y

Figure 4.

Figure 4

(a) Enzymatic reaction carried out in the channel. The fluorescent intensity was detected in the channel with equal distance intervals. (b) The fluorescent intensity at the starting point of reaction (circle) and the point after a certain reaction time (square). The fluorescent intensity at the middle of the curves was used as effective fluorescent intensity in Fig. 4c. (c) Initial reaction rate (v0) curve with substrate concentration ranging from 91 μM to 545 μM. The concentration of enzyme was set as 5 mg/l.