Table 1.
Experimental conditions used for the LC/ESI–MS/MS analysis of polyunsaturated fatty acid-derived prostanoids, hydroxy fatty acids, and various chiral hydroxy fatty acid species.
| Mediator | MRM (m/z) | CE (eV) | RT (min) |
|---|---|---|---|
| (A) Prostanoids | |||
| 15d-PGJ2 | 315→271 | 15 | 18.71 |
| PGJ2 | 333→271 | 15 | 11.08 |
| Δ12-PGJ2 | 333→271 | 15 | 11.99 |
| 15-Keto-PGE2 | 349→113 | 23 | 6.74 |
| PGE3 | 349→269 | 15 | 4.03 |
| PGD3 | 349→269 | 15 | 4.52 |
| PGE2 | 351→271 | 17 | 4.85 |
| PGD2 | 351→271 | 17 | 5.75 |
| PGF2α | 353→193 | 25 | 3.95 |
| PGE1 | 353→317 | 15 | 4.93 |
| PGD1 | 353→317 | 15 | 5.50 |
| PGF1α | 355→311 | 25 | 3.86 |
| TXB3 | 367→169 | 15 | 3.04 |
| TXB2 | 369→169 | 17 | 3.54 |
| 6-Keto-PGF1α | 369→163 | 23 | 2.80 |
| 13,14-dh PGF2α | 355→311 | 25 | 5.18 |
| 13,14-dh-15-k-PGE1 | 353→335 | 12 | 8.87 |
| 13,14-dh-15-k-PGE2 | 351→333 | 12 | 8.05 |
| 13,14-dh-15-k-PGF2α | 353→113 | 28 | 6.90 |
| 13,14-dh-15-k-PGF1α | 355→193 | 32 | 7.64 |
| PGB2-d4 | 337→179 | 20 | 11.99 |
| (B) Chiral hydroxy fatty acid species | |||
| 15-Epi-LXA4 | 351→115 | 20 | 10.97 |
| LXA4 | 351→115 | 20 | 12.22 |
| RvD1 | 375→141 | 15 | 13.43 |
| RvE1 | 349→195 | 17 | 6.55 |
| 18(R)-HEPE | 317→133 | 20 | 26.80 |
| 18(S)-HEPE | 317→133 | 20 | 29.43 |
| 15(R)-HETE | 319→175 | 18 | 45.45 |
| 15(S)-HETE | 319→175 | 18 | 47.89 |
| 17(R)-HDHA | 343→201 | 15 | 54.77 |
| 17(S)-HDHA | 343→201 | 15 | 59.18 |
| 12(R)-HETE | 319→179 | 17 | 51.68 |
| 12(S)-HETE | 319→179 | 17 | 53.06 |
| 12(S)-HETE-d8 | 327→184 | 17 | 51.60 |
| (C) Hydroxy fatty acids | |||
| ±9-HODE | 295→171 | 25 | 15.00 |
| ±13-HODE | 295→195 | 25 | 14.82 |
| ±5-HEPE | 317→115 | 20 | 13.04 |
| ±8-HEPE | 317→155 | 18 | 11.76 |
| ±9-HEPE | 317→149 | 20 | 12.15 |
| ±11-HEPE | 317→167 | 20 | 11.56 |
| ±12-HEPE | 317→179 | 17 | 11.85 |
| ±15-HEPE | 317→175 | 18 | 11.37 |
| ±18-HEPE | 317→133 | 25 | 10.89 |
| ±5-HETE | 319→115 | 20 | 19.38 |
| ±8-HETE | 319→155 | 20 | 17.50 |
| ±9-HETE | 319→123 | 20 | 19.02 |
| ±11-HETE | 319→167 | 20 | 16.70 |
| ±12-HETE | 319→179 | 17 | 17.33 |
| ±15-HETE | 319→175 | 18 | 15.72 |
| ±10-HDHA | 343→153 | 17 | 17.33 |
| ±13-HDHA | 343→193 | 17 | 16.88 |
| ±14-HDHA | 343→161 | 20 | 17.33 |
| ±17-HDHA | 343→201 | 15 | 16.25 |
| ±20-HDHA | 343→241 | 20 | 15.45 |
| 15(S)-HETrE | 321→221 | 15 | 19.47 |
| LTB4 | 335→195 | 17 | 8.39 |
| LTB5 | 333→195 | 20 | 6.43 |
| PD1 | 359→206 | 15 | 5.50 |
| RvD1 | 375→141 | 15 | 4.16 |
| RvE1 | 349→195 | 17 | 1.93 |
| LXA4 | 351→115 | 20 | 4.10 |
| 5-Oxo-ETE | 317→203 | 30 | 20.10 |
| 12(S)-HETE-d8 | 327→184 | 17 | 14.66 |
| ±5(6)-EET | 319→191 | 20 | 23.58 |
| ±8(9)-EET | 319→127 | 22 | 22.24 |
| ±11(12)-EET | 319→208 | 15 | 21.44 |
| ±14(15)-EET | 319→113 | 30 | 19.02 |
| ±5,6-DHET | 337→145 | 25 | 13.39 |
| ±8,9-DHET | 337→127 | 25 | 11.78 |
| ±11,12-DHET | 337→167 | 22 | 11.03 |
| ±14,15-DHET | 337→207 | 25 | 10.22 |
CE, collision energy; RT, indicative retention time.