Table 4.
Protein-protein interactions assayed by Bacterial adenylate cyclase two-hybrid system
| Protein | TpsA | TpsB | TpsC | TppA |
|---|---|---|---|---|
| TpsA |
418 (210–863)* |
1746 (1582–1799) |
113 (77–135) |
71 (43–89) |
| TpsB |
1593 (1467–1832) |
1776 (1658–1988) |
441 (341–560) |
581 (322–714) |
| TpsC |
172 (101–244) |
688 (315–980) |
1214 (861–1551) |
80 (67–102) |
| TppA | 429 (167–656) | 691 (462–987) | 156 (133–198) | 83 (58–98) |
*Estimated values are in units/mg dry weight bacteria. Values in parentheses are the highest and lowest scores for each based on three to four independent assays. The positive control zip-zip (T18 and T25 fragments of the leucine zipper of GCN4) was scored to 3429 (2938–4270). Negative controls and remaining protein interactions scored at maximum 220 (zip-tpsA) but usually less than 50. Values in bold are considered true protein-protein interactions.