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. 2014 Apr 8;14:68. doi: 10.1186/1471-230X-14-68

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Copyright © 2014 Matsumoto et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantification of in vitro transduction of vesicular stomatitis virus G protein (VSV-G)-pseudotyped lentivirus (LV) encoding mouse interleukin 10 (mIL10) in colonic cell lines. Cell line production of mIL-10 in pg/ml following three titered infectious doses of LV (0.1 ng/p24, 1.0 ng/p24, 10 ng/p24) for transfection are shown. Each experiment collected took triplicate supernatant, and measured IL-10 by ELISA. All values were expressed as mean ± SD. *p < 0.05 compared with the results of 0.1 ng p24 LV transduction.