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. 2014 Apr 8;14:68. doi: 10.1186/1471-230X-14-68

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Copyright © 2014 Matsumoto et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Optimization of mucosal LV transfection. Ex vivo Bioluminescence imaging (BLI) analyses. Ex vivo BLI quantification and analyses of GI tract following intrarectal administration of LV expressing firefly luciferase (fLuc) on either colon with (a) PBS only, (b) with PBS followed by 3% DSS, (c) with 20% EtOH only, or (d) with 20% EtOH followed by 3% DSS. This pseudocolor image, superimposed on a gray scale reference image, uses color (blue: least intense; red: most intense) to illustrate signal strength. Abbreviations: Sto: stomach; Si: small intestine; Co: colon; Ce: Cecum; Re: rectum; Li: liver; Ki: kidney; Sp: spleen. (e) quantifies photon emission (p/s/cm2/sr) in the distal colon. *p < 0.05.