Table 1.
Oligonucleotides Used in This Study
| Oligo | 5′–3′ sequence (restriction sites underlined) | Restriction site |
|---|---|---|
| P1 | GCGGCGGCCGCCACCATGGTGATTCTTCAGCAGGGGGAC | NotI |
| P2 | GCGGCTAGCGAAGTTCCGCAGGTACTTGAC | NheI |
| P3 | GCGCTTAAGCAGGTCTAACTTTCTGAAGCTG | AflII |
| P4 | GCGGGTACCTCACTTGCCGCTCCTGGAGCC | KpnI |
| P5 | GGCACCTAGTGGCTTTGAGGTAAGTATCAAGGTTACAAGAC | |
| P6 | GCGGCTAGCTCAGAAACGCAAGAGTCTTC | NheI |
| P7 | CTTCTTTGTGCGATGCATCAAG | NsiI |
| P8 | GCGCTTAAGCGACGCATGCTCGCGATAG | AflII |
| P9 | CGCCCTCGCTCCAGGTCCTGTGGAGAGAAAGGCAAAG | |
| P10 | GAACCCGAACCGGTCCTTG | AgeI |
| P11 | GCGGCTAGCCCCCGGGTGCGCGGCG | NheI |
| P12 | GCGGTCGACGAAACGGTCCAGGCTATGTG | SalI |
| P13 | GCGGCGGCCGCCCCCGGGTGCGCGGCG | NotI |
| P14 | GCGCTTAAGGAAACGGTCCAGGCTATGTG | AflII |
| P15 | CAGGCACCTAGTGGCTTTGAGGTACCAGGCTAGGGACAGG | |
| P16 | GCGGCTAGCCGCCTGAGCCCAGAAGTTC | NheI |
| P17 | CGCCCTCGCTCCAGGTCCTGAAGGAGACAAGAGGTATG | |
| P18 | GCGCTTAAGCACCGCTTGTGTTGATCCTC | AflII |
| P19 | GCCAGGGAAGGATCCCATG | BamHI |
| P20 | GCGGGTACCTCATGCGTAATCCGGTACATCGTAAGGGTACTTGCCGCTCCTGGAGCC | KpnI |
| P21 | AGCTTCGTAGAGTTTGTGGAGCGG | |
| P22 | GAGGGGCAAACAACAGATG |
Oligonucleotides were used to make 5′ and 3′ vectors of the dual-vector platforms (P1–P20). Oligonucleotides were used to characterize the fidelity of the overlap in simple overlap, trans-splicing and AP hybrid vector platforms (P21–P22). Restriction sites used for cloning are underlined and the introduced HA tag is noted in italics (P19).