FIG. 2.
HTMs and hMSCs have similar expression of self-renewal transcription factors but differ in Dex responsiveness. (A) When compared to hMSCs, HTMs express equivalent or higher levels of the self-renewal/pluripotency genes sox2 and pouf5f1. They additionally express similar levels of notch1, a transmembrane receptor known to be expressed by hMSCs and important in differentiation (n=3; representative results shown). (B) The expression of these proteins is not influenced by 10−7 M dexamethasone (Dex) treatment when compared to the vehicle control (EtOH). Data mean±standard error of the mean of cells from 3 (hMSC) and 6 (HTM) donors. (C) hMSCs (donors J, D, and H) myoc and angptl7 expression is far lower than HTM cells and lacks the consistent and robust HTM response to Dex. Significance between EtOH/Dex indicated with *, **, ***. Significance between HTM/hMSC indicated with ###. “n/e” indicates no reliable detectable expression.