FIG. 6.

Aqueous humor does not differentiate hMSCs into HTM cells. (A) Expression of myoc and angptl7 mRNA remained lower than control HTM cells. Additionally, there was a limited and inconsistent response to 100 nM Dex. (B) Pluripotency factors sox2 and pou5f1 were significantly decreased by culture in 20% bovine aqueous humor (AH) but not 100% AH. Expression of notch1 was unaffected by either media. In all cases, 100 nM Dex treatment reduced the expression of sox2 in control media and 100% AH, and notch1 and pou5f1. Significance between EtOH/Dex indicated with *, **, ***. Significant difference from HTM control (for myoc/angptl7) or from control hMSC (for sox2/pou5f1) indicated with ^###. “n/e” indicates no detectable expression. (C) Alkaline phosphatase staining of hMSCs. Minimal staining is observed in control or 100% AH cultured cells, but prominent staining is observed in 20% AH and osteogenic cultures. Scale bar is 50 μm. Color images available online at www.liebertpub.com/jop