FIG. 7.

HTM cells express α-smooth muscle actin (αSMA). (A) Fresh HTM explant cultures stain strongly positive for αSMA, whereas hMSCs have sparse immunoreactivity. (B) HTM explant cultures have higher mRNA expression than transforming growth factor-beta-treated human corneal fibroblasts (HCF+). Untreated human corneal fibroblasts are used as a negative control (HCF−). Significance between groups indicated with ***. (C) The HTM stains weakly positive for αSMA overall. Ciliary muscle exhibits strong immunoreactivity, which is diminished in the posterior meshwork, but the anterior meshwork (C′) and the insert region (C′′) display individually strongly immunoreactive cells. Scale bars are 50 μm. Color images available online at www.liebertpub.com/jop