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. 2013 Dec 12;25(2):115–125. doi: 10.1089/hgtb.2013.131

FIG. 1.

FIG. 1.

(A) Flow diagram depicting the three different PCR-based AAV genome titer assays. (B) Graphical representation of the single-stranded (ssAAV) and self-complementary sc(AAV) vectors used in the study. The line drawings represent the two genome configurations with inverted terminal repeats (ITRs) depicted as lariat structures with the hairpin ITR of the scAAV genome oriented to the left. The lot number of each vector is given below the drawings, and the various TaqMan assays used appear below, with the target sequence position indicated in parentheses. +ve, positive; −ve, negative; BGH, bovine growth hormone; CMV, cytomegalovirus; eGFP, enhanced green fluorescent protein; PF68, Pluronic F-68; PrK, proteinase K; QX-100, QX100 droplet reader; RBG, rabbit β-globin; scAAV, self-complementary AAV; ssAAV, single-stranded AAV; SV40, simian virus 40; TBG, human thyroxine-binding globulin.