Table 1.
Plasmid Standard Copy Number Validation by Droplet Digital PCR
| Plasmid DNA standard | Calculated copiesa | Assay | ddPCR-detected copies |
|---|---|---|---|
| RBG (pAAV.CBA.PI.eGFP.RBG) | 1,000 | nRBG | 488 |
| 1,000 | eGFP | 502 | |
| 10,000 | nRBG | 6,920b | |
| 10,000 | nRBG | 6,313b | |
| 1,000 | nRBG | 677b | |
| BGH (pAAV.CMV.eGFP.BGH) | 1,000 | CMV2 | 1,096b |
| 1,000 | eGFP | 1,014b | |
| 1,000 | eGFP | 1,063b | |
| 10,000 | eGFP | 11,373b |
a
Determined by spectrophotometry.
b
Modified PCR cycling conditions.
BGH, bovine growth hormone poly(A); CMV, cytomegalovirus promoter; ddPCR, droplet digital PCR; eGFP, enhanced green fluorescent protein; RGB, rabbit β-globin poly(A).