Table 2.
Lot no. | Assay | ddPCR | OqPCR | ddPCR: OqPCR | ddPCR: Distal: Proximala | OqPCR: Distal: Proximala | |
---|---|---|---|---|---|---|---|
ss vectors | CS0008 | CMV2 | 7.04×1012 | ND | N/A | ||
eGFP | 6.75×1012 | ND | N/A | 0.96 | N/A | ||
nRBG | 6.31×1012 | 7.22×1012 | 0.87 | 0.90 | N/A | ||
WL057CS | TBG | 3.84×1013 | 3.93×1013 | 0.98 | |||
nRBG | 3.01×1013 | 3.06×1013 | 0.98 | 0.78 | 0.78 | ||
WL705S | TBG | 3.39×1013 | ND | N/A | |||
BGH | 3.68×1013 | 3.74×1013 | 0.98 | 1.09 | N/A | ||
WL711S | TBG | 4.96×1013 | ND | N/A | |||
BGH | 5.26×1013 | 4.80×1013 | 1.10 | 1.06 | N/A | ||
sc vectors | CS0036 | CMV | 4.36×1012 | 2.27×1012 | 1.92 | ||
eGFP | 8.68×1012 | 5.22×1012 | 1.66 | 1.99 | 2.30 | ||
nRBG | 8.00×1012 | 5.30×1012 | 1.51 | 1.83 | 2.34 | ||
V1258 | CMV | 3.44×1012 | 1.61×1012 | 2.14 | |||
eGFP | 5.87×1012 | 3.00×1012 | 1.96 | 1.71 | 1.86 | ||
nRBG | 5.46×1012 | 3.37×1012 | 1.62 | 1.59 | 2.09 | ||
V1306 | CMV | 6.65×1012 | 2.93×1012 | 2.27 | |||
eGFP | 1.14×1013 | 4.79×1012 | 2.39 | 1.72 | 1.63 | ||
nRBG | 9.97×1012 | 4.02×1012 | 2.48 | 1.50 | 1.37 | ||
V1450 | CMV | 1.10×1013 | 5.18×1012 | 2.12 | |||
eGFP | 1.10×1013 | 6.48×1012 | 1.69 | 1.00 | 1.25 | ||
nRBG | 1.09×1013 | 7.35×1012 | 1.48 | 0.99 | 1.42 | ||
WL005 | TBG | 1.85×1013 | 1.11×1013 | 1.67 | |||
eGFP | 4.60×1013 | 2.17×1013 | 2.11 | 2.48 | 1.97 | ||
SV40 | 4.74×1013 | 1.99×1013 | 2.38 | 2.56 | 1.80 | ||
WL426-7 | TBG | 4.32×1013 | 2.04×1013 | 2.12 | |||
eGFP | 6.45×1013 | 3.93×1013 | 1.64 | 1.49 | 1.93 | ||
SV40 | 6.74×1013 | 3.10×1013 | 2.17 | 1.56 | 1.52 |
Ratio of titers determined by distal assays [transgene/poly(A) signal] and proximal assay (promoter).
BGH, bovine growth hormone poly(A); CMW, cytomegalovirus promoter; ddPCR, droplet digital PCR; eGFP, enhanced green fluorescent protein; OqPCR, optimized qPCR; N/A, not applicable; ND, not determined; nRBG, normal rabbit β-globin poly(A); sc, self-complementary; ss, single-stranded; SV40, simian virus 40 poly(A); TBG, human thyroxine-binding globulin promoter.