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. 2013 Dec 12;25(2):115–125. doi: 10.1089/hgtb.2013.131

Table 2.

TaqMan Assay Comparison

  Lot no. Assay ddPCR OqPCR ddPCR: OqPCR ddPCR: Distal: Proximala OqPCR: Distal: Proximala
ss vectors CS0008 CMV2 7.04×1012 ND N/A    
    eGFP 6.75×1012 ND N/A 0.96 N/A
    nRBG 6.31×1012 7.22×1012 0.87 0.90 N/A
  WL057CS TBG 3.84×1013 3.93×1013 0.98    
    nRBG 3.01×1013 3.06×1013 0.98 0.78 0.78
  WL705S TBG 3.39×1013 ND N/A    
    BGH 3.68×1013 3.74×1013 0.98 1.09 N/A
  WL711S TBG 4.96×1013 ND N/A    
    BGH 5.26×1013 4.80×1013 1.10 1.06 N/A
sc vectors CS0036 CMV 4.36×1012 2.27×1012 1.92    
    eGFP 8.68×1012 5.22×1012 1.66 1.99 2.30
    nRBG 8.00×1012 5.30×1012 1.51 1.83 2.34
  V1258 CMV 3.44×1012 1.61×1012 2.14    
    eGFP 5.87×1012 3.00×1012 1.96 1.71 1.86
    nRBG 5.46×1012 3.37×1012 1.62 1.59 2.09
  V1306 CMV 6.65×1012 2.93×1012 2.27    
    eGFP 1.14×1013 4.79×1012 2.39 1.72 1.63
    nRBG 9.97×1012 4.02×1012 2.48 1.50 1.37
  V1450 CMV 1.10×1013 5.18×1012 2.12    
    eGFP 1.10×1013 6.48×1012 1.69 1.00 1.25
    nRBG 1.09×1013 7.35×1012 1.48 0.99 1.42
  WL005 TBG 1.85×1013 1.11×1013 1.67    
    eGFP 4.60×1013 2.17×1013 2.11 2.48 1.97
    SV40 4.74×1013 1.99×1013 2.38 2.56 1.80
  WL426-7 TBG 4.32×1013 2.04×1013 2.12    
    eGFP 6.45×1013 3.93×1013 1.64 1.49 1.93
    SV40 6.74×1013 3.10×1013 2.17 1.56 1.52
a

Ratio of titers determined by distal assays [transgene/poly(A) signal] and proximal assay (promoter).

BGH, bovine growth hormone poly(A); CMW, cytomegalovirus promoter; ddPCR, droplet digital PCR; eGFP, enhanced green fluorescent protein; OqPCR, optimized qPCR; N/A, not applicable; ND, not determined; nRBG, normal rabbit β-globin poly(A); sc, self-complementary; ss, single-stranded; SV40, simian virus 40 poly(A); TBG, human thyroxine-binding globulin promoter.