Table 3.
na | Assay | Median CVb | CV range | |
---|---|---|---|---|
Optimized qPCR | 36 | nRBG | 5.35 | 2.11–33.23 |
17 | BGH | 4.37 | 1.95–8.72 | |
ddPCR | 4 | nRBG | 2.21 | 0.60–2.84 |
7 | BGH | 1.65 | 0.57–2.19 | |
13 | eGFP | 1.81 | 1.22–5.01 |
Independent assays performed in triplicate.
Median of triplicate CV values.
BGH, bovine growth hormone poly(A); CV, coefficient of variation; ddPCR, droplet digital PCR; eGFP, enhanced green fluorescent protein; nRBG, normal rabbit β-globin poly(A).