Table 3.
Intraassay Precision of Optimized qPCR and Droplet Digital PCR Assays
| na | Assay | Median CVb | CV range | |
|---|---|---|---|---|
| Optimized qPCR | 36 | nRBG | 5.35 | 2.11–33.23 |
| 17 | BGH | 4.37 | 1.95–8.72 | |
| ddPCR | 4 | nRBG | 2.21 | 0.60–2.84 |
| 7 | BGH | 1.65 | 0.57–2.19 | |
| 13 | eGFP | 1.81 | 1.22–5.01 |
a
Independent assays performed in triplicate.
b
Median of triplicate CV values.
BGH, bovine growth hormone poly(A); CV, coefficient of variation; ddPCR, droplet digital PCR; eGFP, enhanced green fluorescent protein; nRBG, normal rabbit β-globin poly(A).