Table 4.

Interassay Precision

	na	Assay	Sample dilution	Mean (GC/ml)	SD	CV
Standard qPCR	13	RBG	1×	1.23×1013	2.03×1012	16.50
Optimized qPCR	6	RBG	1×	3.06×1013	4.44×1012	14.50
			100×	2.75×1013	3.53×1012	12.80
			1000×	2.77×1013	4.57×1012	16.50
	6	TBG	1×	3.93×1013	4.53×1012	11.50
			100×	3.52×1013	4.52×1012	12.80
			1000×	3.74×1013	5.31×1012	14.20
ddPCR	4	RBG	1×	3.01×1013	1.64×1012	5.45
	5		10×	2.97×1013	1.01×1012	3.38
	5		100×	2.69×1013	8.40×1011	3.12
	5		1000×	2.67×1013	2.12×1012	7.92
	4	TBG	1×	3.84×1013	1.95×1012	5.08
	5		10×	3.85×1013	2.81×1012	7.29
	5		100×	3.46×1013	9.30×1011	2.69
	5		1000×	3.59×1013	1.77×1012	4.93

^aNumber of independent assays performed with ssAAV vector lot number WL057CS.

CV, coefficient of variation; ddPCR, droplet digital PCR; GC, genome copies; RBG, rabbit β-globin poly(A); TBG, human thyroxine-binding globulin promoter.