Table 4.
na | Assay | Sample dilution | Mean (GC/ml) | SD | CV | |
---|---|---|---|---|---|---|
Standard qPCR | 13 | RBG | 1× | 1.23×1013 | 2.03×1012 | 16.50 |
Optimized qPCR | 6 | RBG | 1× | 3.06×1013 | 4.44×1012 | 14.50 |
100× | 2.75×1013 | 3.53×1012 | 12.80 | |||
1000× | 2.77×1013 | 4.57×1012 | 16.50 | |||
6 | TBG | 1× | 3.93×1013 | 4.53×1012 | 11.50 | |
100× | 3.52×1013 | 4.52×1012 | 12.80 | |||
1000× | 3.74×1013 | 5.31×1012 | 14.20 | |||
ddPCR | 4 | RBG | 1× | 3.01×1013 | 1.64×1012 | 5.45 |
5 | 10× | 2.97×1013 | 1.01×1012 | 3.38 | ||
5 | 100× | 2.69×1013 | 8.40×1011 | 3.12 | ||
5 | 1000× | 2.67×1013 | 2.12×1012 | 7.92 | ||
4 | TBG | 1× | 3.84×1013 | 1.95×1012 | 5.08 | |
5 | 10× | 3.85×1013 | 2.81×1012 | 7.29 | ||
5 | 100× | 3.46×1013 | 9.30×1011 | 2.69 | ||
5 | 1000× | 3.59×1013 | 1.77×1012 | 4.93 |
Number of independent assays performed with ssAAV vector lot number WL057CS.
CV, coefficient of variation; ddPCR, droplet digital PCR; GC, genome copies; RBG, rabbit β-globin poly(A); TBG, human thyroxine-binding globulin promoter.