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Bulk Preparation Interference with Droplet Digital PCR and Optimized qPCR Assays
| log10 bulk vector dilution | ||||||||
|---|---|---|---|---|---|---|---|---|
| 0 | −1 | −2 | −3 | −4 | −5 | −6 | −7 | |
| Input bulk vector copies | 1.5×1010 | 1.5×109 | 1.5×108 | 1.5×107 | 1.5×106 | 1.5×105 | 1.5×104 | 1.5×103 |
| Spike copies | 3.5×103 | 3.5×103 | 3.5×103 | 3.5×103 | 3.5×103 | 3.5×103 | 3.5×103 | 3.5×103 |
| ddPCR % spike recovery | 1.0 | 0.0 | 3.0 | 110.0 | 112.0 | 116.0 | 118.0 | 111.0 |
| OqPCR % spike recovery | 0.0 | 0.0 | 3.6 | 4.3 | 78.0 | 116.0 | 115.0 | 111.0 |
ddPCR, droplet digital PCR; OqPCR, optimized qPCR.
