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. 2014 Mar 1;30(2-3):191–201. doi: 10.1089/jop.2013.0190

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 7. — Schlemm's canal (SC) and juxtacanalicular meshwork (JCT) interface. Orthogonal reconstruction (A) and tangential views (B–D) of interface region of JCT/inner wall endothelium next to SC lumen. (A) Orthogonal view through JCT/inner wall region, next to SC signal void (between dotted lines) region (b: JCT/inner wall interface; c: inner wall nuclei; d: SC lumen). (B) Fine autofluorescent fibers (arrows) are seen among Hoechst-labeled nuclei in the JCT region. Region of SC endothelium should have nuclei only without autofluorescent fibers (darker background). (C) SC signal void in a more external optical section just beyond JCT. (D) Autofluorescence signal from nylon marker (asterisk) in SC lumen showing course of SC without exogenous labeling. Arrowheads: Hoechst-labeled nuclei. Scale bar=25 μm.