FIG. 5.
Maintenance of CAG promoter-controlled transgene expression on differentiation is transgene line dependent. (A) Phase-contrast and fluorescence microscopy images of eGFP expression in the transgenic cell line hCBiPS2 CAG-eGFP2 at the undifferentiated stage (left), after spontaneous differentiation induced by EB-formation on day 7 (middle), and in an EB outgrowth on day 14 (right). Scale bars: 100 μm. (B) Graph depicting the quantitative analysis of three independent cell lines at the same respective differentiation stages by flow cytometry, revealed a general but differential, cell line-dependent decrease in the proportion of eGFPpos cells (“silencing” of transgene expression). Color images available online at www.liebertpub.com/hgtb