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. 2013 Nov 24;(81):50465. doi: 10.3791/50465
glu. (μg) 0 0.04 0.08 0.12 0.16 0.2
Raw Data Fluorescence (RDF) fluo. 60 88.8 106.7 121.8 148 172.1
RDF - Background (fluorescence for 0 μg in standard curve) correction 0 28.8 46.7 61.8 88 112.1
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Raw Data Fluo. (RDF) RDF - Background (correction*1000) / slope standard curve Volume of sample used to titrate glycogen Raw data protein concen-tration γ * volume sample Glucose (ng) / protein total
Total glucose concentration 1 fluo. correction glucose (ng) volume sample γ (μg/μl) protein total glucose (ng/μg Pr.)
Sample 1 (CCL39-NX 1) 65.8 5.8 10.5 20 0.07 1.4 7.5
Sample 2 (CCL39-NX 2) 64.1 4.1 7.4 20 0.11 2.2 3.4
Sample 3 (CCL 39 HX 48 hr 1) 177.3 117.3 211.5 4 0.22 0.88 240.3