Figure 1.

Phenols extract from olive vegetation water effects on E. coli K-12.The antimicrobial effect of PEOVW seems related to direct envelope damage. The cell responded by increasing the transcription of genes encoding enzymes for the repair of nucleic acids and antioxidant enzymes. In addition, the bacteria responded with a general energy conservation strategy by decreasing the TCA cycle, glicolysis and motility. Bacterial motility plays a pivotal role in microbial surface colonization and the spreading of bacteria across the surface, while it is usually dispensable for the initial stages of biofilm development. In addition, the depressive effect of PEOVW on the expression of several genes involved in the synthesis of fimbriae, curli and exopolysaccharides suggests that PEOVW can modify the adhesion to the surface. Many key genes (bhsA, csgC, rcsA, bssS, bssR, ydaM, yddV, yhjH) involved in biofilm formation and regulation were differently expressed.