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. 2014 Apr 9;176(2):238–245. doi: 10.1111/cei.12275

Figure 4.

Figure 4

Caspase recruitment domain-containing protein 11 (CARD11) silencing inhibited CARD11/Bcl110 assembly and nuclear factor (NF)-κB activation. (a,b) CARD11 protein was reduced by 80% by siRNA treatment, as measured by Western blotting. (c) Bcl10 was immunoprecipitated (IP) from cell lysates. The resulting immunopurified protein complexes were subjected to immunoblot analyses (IB) with the indicated antibodies. To determine the cellular levels of the CARD11/Bcl110 complex, control immunoblot analyses were performed with the indicated antibodies. (d) CARD11 silencing inhibited NF-κB activation. (e) Analysis of band intensity of Western blot. Values are the mean ± standard deviation (s.d.). #P < 0·01 versus normal control; **P < 0·01 versus control siRNA-treated group; n = eight mice per group.