Figure 5.

GDAs^BMP express high levels of thrombospondin and increase synaptophysin expression in 6-OHDA-lesioned rats.

A Relative fluorescence intensity of synaptophysin staining in injured striatum of 6-OHDA injured animals after treatment with saline (placebo) or hGDAs^BMP shows rescue to levels seen uninjured tissue by GDA^BMP transplantation. Expression was normalized to synaptophysin staining in contralateral, uninjured striatum. Mean of percentage ± s.d., n = 3. *P < 0.05, Unpaired t-test.

B, C Gene expression analysis of Thrombospondin 1 and 2 by semi-quantitative RT-PCR shows markedly higher levels of expression in GDAs^BMP than in GRP cells or GDAs^CNTF. Average relative expression normalized to expression found in glial precursors. Mean of n = 3 ± s.d., *P < 0.05 for all pairwise comparisons by One-Way ANOVA and Bonferroni Multiple Comparison Test except for comparison of GRP and GDA^CNTF conditions (ns).