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. 2014 Feb 27;6(4):551–566. doi: 10.1002/emmm.201303356

Figure 3.

Figure 3

Increasing GABA levels endogenously also inhibits pexophagy and mitophagy, and these defects are suppressed by rapamycin.
  1. WT cells expressing OM45-GFP, along with the uga2Δ strain over-expressing the GAD1 gene and expressing OM45-GFP were grown in YPL medium to mid-log-phase. To monitor mitophagy, strains were transferred to SD-N starvation medium (with or without rapamycin).
  2. WT strain along with the uga2Δ strain over-expressing the GAD1 gene was grown in oleate medium and pexophagy was monitored as described in Fig 1, with or without rapamycin. Samples were taken at the indicated time points, and Pot1 degradation was analyzed by immunoblotting (45 kD).
  3. To monitor autophagy, WT cells expressing GFP-Atg8 along with the uga2Δ strain over-expressing the GAD1 gene and expressing GFP-Atg8 were grown in SD medium and transferred to SD-N.