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The combined experimental paradigm using a rodent hypoxic and brain slice model to investigate the cellular effects of preoperative hypoxia on WM oligodendrocyte injury. (a) Study design and equivalent time period for WM development between the mouse and human. (b) Perfusion protocol for oxygen-glucose deprivation under hypothermia. (c) Antibody markers used to immunostain distinct developmental stages of oligodendrocyte lineage cells.
