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. Author manuscript; available in PMC: 2015 May 1.

Published in final edited form as: Gastroenterology. 2014 Feb 4;146(5):1289–1300.e19. doi: 10.1053/j.gastro.2014.01.056

Oral administration of scCD98-functionalized siCD98-loaded NPs decrease acute colitis induced by DSS. (A) Mouse body weight over time, normalized as a percentage of day zero body weight and given as the mean of each treatment group. (B) Colonic myeloperoxidase (MPO) activity. The results are expressed as units of MPO activity per milligram of protein. (C) The mRNA levels of CD98 and pro-inflammatory cytokines in mice with different treatments. Each point represents the mean ± S.E.M. (n=5). Statistical significance was assessed using the Student’s t-test (*P<0.05 and **P<0.01). (D) Representative H&E-stained colon sections from DSS-treated mice administered with daily double gavages of hydrogel with or without NPs for 4 days. Scale bar represents 100 μm. (E) Representative FCM analysis and (F) percentage of FITC fluorescence-positive colon epithelial cells and macrophages in mice receiving DSS and treated with hydrogel with or without scCD98-functionalized FITC-siRNA-loaded NPs. Data are expressed as mean ± S.E.M. (n=3). Statistical significance was performed using Student’s t-test (*P<0.05 and **P<0.01).

Oral administration of scCD98-functionalized siCD98-loaded NPs decrease acute colitis induced by DSS. (A) Mouse body weight over time, normalized as a percentage of day zero body weight and given as the mean of each treatment group. (B) Colonic myeloperoxidase (MPO) activity. The results are expressed as units of MPO activity per milligram of protein. (C) The mRNA levels of CD98 and pro-inflammatory cytokines in mice with different treatments. Each point represents the mean ± S.E.M. (n=5). Statistical significance was assessed using the Student’s t-test (*P<0.05 and **P<0.01). (D) Representative H&E-stained colon sections from DSS-treated mice administered with daily double gavages of hydrogel with or without NPs for 4 days. Scale bar represents 100 μm. (E) Representative FCM analysis and (F) percentage of FITC fluorescence-positive colon epithelial cells and macrophages in mice receiving DSS and treated with hydrogel with or without scCD98-functionalized FITC-siRNA-loaded NPs. Data are expressed as mean ± S.E.M. (n=3). Statistical significance was performed using Student’s t-test (*P<0.05 and **P<0.01).