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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Gastroenterology. 2014 Feb 8;146(5):1373–1385.e11. doi: 10.1053/j.gastro.2014.02.002

Figure 4. OSBP inhibition alters web morphology without blocking HCV-induced PI(4)P accumulation.

Figure 4

(A) Nonreplicative expression of the NS3-5B(NS5A/GFP) polyprotein in Huh7.5.1/T7 cells stably expressing T7 RNA polymerase and the indicated shRNAs. 48 hr post-transfection, cells were immunostained for PI(4)P (red) and GFP (green) with DAPI nuclear counterstaining (blue). Arrowheads indicate cells expressing the NS3-5B/GFP polyprotein. Bar, 20 μm.

(B) Quantitation of PI(4)P fluorescence from HCV-expressing cells (open symbols) versus HCV-nonexpressing cells (closed symbols). Each point denotes the integrated fluorescence signal from a single cell with the mean fluorescence indicated by the black lines.

(C) Immunostaining of JFH-1 infected Huh7.5.1 cells stably expressing the indicated shRNAs for PI(4)P (red) and GFP (green) with DAPI nuclear counterstaining (blue). Pr, Pearson’s correlation coefficients; bar, 10 μm.