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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Gastroenterology. 2014 Feb 8;146(5):1373–1385.e11. doi: 10.1053/j.gastro.2014.02.002

Figure 7. The dependence of RNA viruses on OSBP correlates with their dependence on PI 4-kinases.

Figure 7

(A) Huh7.5.1 cells containing a subgenomic DENV replicon expressing a Renilla luciferase reporter were transduced with the indicated shRNAs for 72 hr prior to luciferase activity measurement (left panel) and immunoblotting (right panel). Values (means ± SD, two independent experiments) are normalized to control cells

(B) DENV replicon cells were treated with the indicated concentrations of OSW-1 (left panel) or AL-9 (right panel) for 24 hr before luciferase activity measurement. Values are means ± SD, two independent experiments.

(C) HeLa cells stably expressing a nontargeting shRNA or three independent OSBP shRNAs were infected with poliovirus (MOI 0.1) 24 hr before measurement of viral titers (left panel), and immunoblotting (right panel). *, P = .004 and **, P< .001 versus nontargeting shRNA.