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. Author manuscript; available in PMC: 2014 Apr 20.
Published in final edited form as: Microsc Microanal. 2013 Feb;19(1):201–212. doi: 10.1017/S1431927612014080

Figure 5.

Figure 5

Figure 5

a: Intensity ratios of confocal fluorescence vs. two-photon excited fluorescence for DsRed2 as a function of penetration depth. Attenuation difference is 21cm−1 giving TPEF an intensity advantage of ~2 at a depth of 320 microns.

b: Intensity ratios of confocal fluorescence vs. two-photon excited fluorescence for eGFP as a function of penetration depth (only cells 100 micron below the sample surface were analyzed). Data was obtained on an LSM510 with 40× NA:1.0 WI objective lens. TPEF signal advantage is approximately twice that of DsRed2.